Subtract baseline from absorption spectrum

Baselines of pure solvent should always be subtracted from the measured absorption spectrum before evaluating the spectral appearance or before quantitative interpretation of the spectrum. a|e provides an easy way of handling multiple measured baselines, e.g. if absorption spectra are acquired in many different solvents or under different conditions.

1. Load the absorption spectra into the 'Absorption spectra' data listbox and the baselines into the 'Baselines' data listbox.

2. Press the 'Subtract' button located above the Baselines listbox.

3. This will open a selection dialog box. In the right listbox you select the baseline to subtract from all the absorption spectra selected in the left listbox. Press OK.

The baseline has now been subtracted from the selected absorption spectra: